Immunohistochemical properties and spinal connections of pelvic autonomic neurons that innervate the rat prostate gland

Kepper M. and Keast J. (1995) Immunohistochemical properties and spinal connections of pelvic autonomic neurons that innervate the rat prostate gland. Cell & Tissue Research, 281 3: 533-542. doi:10.1007/BF00417871


Author Kepper M.
Keast J.
Title Immunohistochemical properties and spinal connections of pelvic autonomic neurons that innervate the rat prostate gland
Journal name Cell & Tissue Research   Check publisher's open access policy
ISSN 0302-766X
Publication date 1995-01-01
Sub-type Article (original research)
DOI 10.1007/BF00417871
Volume 281
Issue 3
Start page 533
End page 542
Total pages 10
Publisher Springer-Verlag
Subject 2702 Anatomy
1308 Clinical Biochemistry
1307 Cell Biology
Abstract Autonomic innervation of the prostate gland supplies the acini, and non-vascular and vascular smooth muscle. The activity of each of these tissues is enhanced by sympathetic outflow, whereas the role of the parasympathetic nervous system in this organ is unclear. In the present study, a range of methods was applied in rats to determine the location of autonomic neurons supplying this gland, the immunohistochemical properties of these neurons, the spinal connections made with the postganglionic pathways and the distribution of various axon types within the gland. Injection of the retrograde tracer, FluoroGold, into the ventral gland visualised neurons within the major pelvic ganglion and sympathetic chain. Fluorescence immunohistochemical studies on the labelled pelvic neurons showed that most were noradrenergic (also containing neuropeptide Y, NPY), the others being non-noradrenergic and containing either vasoactive intestinal peptide (VIP) or NPY. Sympathetic dyelabelled neurons were identified by the presence of varicose nerve terminals stained for synaptophysin on their somata following lesion of sacral inputs. Parasympathetic innervation of dye-labelled neurons was identified by continued innervation after hypogastric nerve lesion. Most noradrenergic prostate-projecting neurons were sympathetic, as were many of the non-noradrenergic VIP neurons. Parasympathetic prostate-projecting neurons were largely non-noradrenergic and contained either VIP or NPY. All substances found in retrogradely labelled somata were located in axons within the prostate gland but had slightly different patterns of distribution. The studies have shown that there are a significant number of non-noradrenergic sympathetic prostate-projecting neurons, which contain VIP.
Keyword FluoroGold
Neuropeptides
Pelvic plexus
Rat (Wistar)
Reproductive tract, male
Retrograde tracing
Synaptophysin
Tyrosine hydroxylase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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