Competence for gene transfer by electroporation in a sub-population of protoplasts from uniform carrot cell suspension cultures

Bower R. and Birch R.G. (1990) Competence for gene transfer by electroporation in a sub-population of protoplasts from uniform carrot cell suspension cultures. Plant Cell Reports, 9 7: 386-389. doi:10.1007/BF00232405


Author Bower R.
Birch R.G.
Title Competence for gene transfer by electroporation in a sub-population of protoplasts from uniform carrot cell suspension cultures
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 1990-01-01
Sub-type Article (original research)
DOI 10.1007/BF00232405
Open Access Status Not yet assessed
Volume 9
Issue 7
Start page 386
End page 389
Total pages 4
Publisher Springer-Verlag
Subject 1110 Nursing
Abstract We have investigated the basis for increased transient reporter gene expression following electroporation of protoplasts from uniform carrot cell suspension cultures at increasing DNA concentrations. Use of a combination of histochemical and fluorometric GUS gene assays allowed differentiation between increases due to a higher proportion of expressing protoplasts and increases due to higher expression by each expressing protoplast. A plateau of 20-25% expressing protoplasts was reached by 50 μg ml-1 DNA but total expression continued to increase in direct proportion to applied DNA concentration up to at least 100 μg ml-1. This indicates the existence of a subpopulation of protoplasts competent for the uptake and expression of genes by electroporation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 30 Aug 2016, 11:42:56 EST by System User