Immuno-dot blot as a rapid diagnostic method for detection of chlamydial infection in koalas (Phasolarctos cinereus).

Girjes A.A., Ellis W.A., Lavin M.F. and Carrick F.N. (1993) Immuno-dot blot as a rapid diagnostic method for detection of chlamydial infection in koalas (Phasolarctos cinereus).. Veterinary Record, 133 6: 136-141.

Author Girjes A.A.
Ellis W.A.
Lavin M.F.
Carrick F.N.
Title Immuno-dot blot as a rapid diagnostic method for detection of chlamydial infection in koalas (Phasolarctos cinereus).
Journal name Veterinary Record   Check publisher's open access policy
ISSN 0042-4900
Publication date 1993-08-07
Year available 1993
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 133
Issue 6
Start page 136
End page 141
Total pages 6
Place of publication LONDON
Publisher BRITISH VETERINARY ASSOC
Language eng
Subject 3400 Veterinary
Abstract The sensitivity and specificity of an immunoscreening test for anti-chlamydial antibodies in koala (Phasolarctos cinereus) serum were determined after the adsorption of non-specific antibodies. The results of the test were compared with complement fixation tests, tissue culture, gene probe analysis and dot blot immunoscreening for host-borne chlamydial antigen. The immunoscreening test was the most sensitive test for the identification of chlamydial infection in koala serum samples, furthermore it was rapid, taking approximately 16 hours to complete, and inexpensive. However, for the assay of swab material from koalas, gene probe analysis remains the most sensitive method of detection of chlamydiae.
Keyword Phascolarctos-Cinereus
Dna Probe
Monoclonal-Antibody
Enzyme-Immunoassay
Tissue-Culture
Psittaci
Trachomatis
Hybridization
Bacteria
Protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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