The rosette inhibition test: Technical considerations

Morton H. and Clunie G.J.A. (1972) The rosette inhibition test: Technical considerations. Transplantation, 14 2: 211-215.

Author Morton H.
Clunie G.J.A.
Title The rosette inhibition test: Technical considerations
Journal name Transplantation   Check publisher's open access policy
ISSN 0041-1337
Publication date 1972-01-01
Sub-type Article (original research)
Volume 14
Issue 2
Start page 211
End page 215
Total pages 5
Subject 2747 Transplantation
2403 Immunology
Abstract The formation of rosettes between spleen cells from nonimmunized mice and sheep red blood cells (SRBC) has been investigated. It has been shown that the number of rosette-forming cells present is greatly increased in the presence of calcium and megnesium and in the higher pH ranges, but decreases rapidly during incubation. When testing antilymphocytic sera to determine the degree of inhibition of rosette formation, ionic concentration, pH, and time and temperature of incubation must be carefully controlled to obtain reproducible results, and it is recommended that tests should be performed at 37 C using Hanks’ basic salt solution (BSS) without calcium or magnesium, pH 7.4, as the suspending medium and that readings should be taken after 1½ hr of incubation.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 23 Aug 2016, 15:30:24 EST by System User