Measurement of binding constants by capillary electrophoresis

Winzor D.J. (1995) Measurement of binding constants by capillary electrophoresis. Journal of Chromatography A, 696 1: 160-163. doi:10.1016/0021-9673(95)00097-7


Author Winzor D.J.
Title Measurement of binding constants by capillary electrophoresis
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
Publication date 1995-04-07
Sub-type Article (original research)
DOI 10.1016/0021-9673(95)00097-7
Volume 696
Issue 1
Start page 160
End page 163
Total pages 4
Subject 1303 Specialist Studies in Education
1602 Criminology
1605 Policy and Administration
Abstract The assumptions inherent in a capillary electrophoresis procedure for evaluating binding constants for interactions between lectins and charged polysaccharides [S. Honda et al., J. Chromatogr., 597 (1992) 377] have been reappraised. Whereas the results were originally interpreted on the basis that the lectin-carbohydrate interaction was restricted to 1:1 complex formation, a more plausible interpretation is shown to be that an approximately constant incremental difference separates the mobilities of the successive complexes formed as the result of saccharide binding to equivalent and independent sites on the lectin. The parameter that is determined by capillary electrophoresis should thus be regarded as the intrinsic binding constant.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 23 Aug 2016, 14:55:02 EST by System User