Adsorption of viruses on magnetic particles-II. Degradation of MS2 bacteriophage by adsorption to magnetite

Atherton J.G. and Bell S.S. (1983) Adsorption of viruses on magnetic particles-II. Degradation of MS2 bacteriophage by adsorption to magnetite. Water Research, 17 8: 949-953. doi:10.1016/0043-1354(83)90171-9


Author Atherton J.G.
Bell S.S.
Title Adsorption of viruses on magnetic particles-II. Degradation of MS2 bacteriophage by adsorption to magnetite
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
Publication date 1983-01-01
Sub-type Article (original research)
DOI 10.1016/0043-1354(83)90171-9
Open Access Status
Volume 17
Issue 8
Start page 949
End page 953
Total pages 5
Subject 1904 Performing Arts and Creative Writing
Abstract The adsorption by magnetite of MS2 bacteriophage from 100 mg 1-1 NaCl solutions was studied using MS2 bacteriophage labelled with 32P in the RNA and 3H in the protein. After agitation for 20 min, the supernatant from suspensions of finely-divided magnetite and MS2 phage in 100 mg 1-1 NaCl, was assayed for remaining infective particles. Phage adsorption to magnetite was shown to occur over the range pH 4-7.5. At phH 6, 99.99% of the MS2 particles were removed from the supernatant and 32P and 3H were associated with the magnetite. When this magnetite was resuspended in 100 mg 1-1 NaCl at pH 10. 32P and 3H was released into the supernatant, although infectious MS2 particles could not be detected. Extracted, purified MS2 RNA labelled with 32P and MS2 protein labelled with 3H were used to study the adsorption and release of the virus components. Both the RNA and protein were bound at pH 6. When the pH was raised to 10, all 32P and 96% of the 3H was released into the supernatant. It is concluded that virus adsorbed to magnetite is disrupted at pH 10, and its components released into the medium. The free virus retains its infectivity at pH 10.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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