Cloning and expression of an Aeromonas hydrophila chitinase gene in escherichia coli

Roffey P.E. and Pemberton J.M. (1990) Cloning and expression of an Aeromonas hydrophila chitinase gene in escherichia coli. Current Microbiology, 21 5: 329-337. doi:10.1007/BF02092100


Author Roffey P.E.
Pemberton J.M.
Title Cloning and expression of an Aeromonas hydrophila chitinase gene in escherichia coli
Journal name Current Microbiology   Check publisher's open access policy
ISSN 0343-8651
Publication date 1990-01-01
Sub-type Article (original research)
DOI 10.1007/BF02092100
Open Access Status
Volume 21
Issue 5
Start page 329
End page 337
Total pages 9
Publisher Springer-Verlag
Subject 2404 Microbiology
Abstract The gene encoding an extracellular chitinase from Aeromonas hydrophila has been cloned and expressed in Escherichia coli. Plasmid pJP2512 contained the smallest DNA insert (3.9 kb) producing chitinase. The chitinase gene is transcribed from its own promoter, producing a protein of Mr 96,000. The chitinase open reading frame, an estimated 2.6 kb in length, has been subcloned to a 3.0 kb fragment; however, this fragment does not carry the functional chitinase promoter. In E. coli the chitinase enzyme is unable to transverse the outer membrane, being secreted across the cytoplasmic membrane and accumulating in the periplasmic space.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 23 Aug 2016, 14:04:46 EST by System User