Some factors affecting the extraneuronal accumulation of adrenaline in guinea-pig trachealis smooth muscle cells

Bryan L.J. and O'Donnell S.R. (1984) Some factors affecting the extraneuronal accumulation of adrenaline in guinea-pig trachealis smooth muscle cells. Naunyn-Schmiedeberg's Archives of Pharmacology, 326 3: 268-272. doi:10.1007/BF00505329


Author Bryan L.J.
O'Donnell S.R.
Title Some factors affecting the extraneuronal accumulation of adrenaline in guinea-pig trachealis smooth muscle cells
Journal name Naunyn-Schmiedeberg's Archives of Pharmacology   Check publisher's open access policy
ISSN 0028-1298
Publication date 1984-01-01
Sub-type Article (original research)
DOI 10.1007/BF00505329
Volume 326
Issue 3
Start page 268
End page 272
Total pages 5
Publisher Springer-Verlag
Subject 3004 Pharmacology
Abstract 1. Tracheal segments from guinea-pigs pretreated with 6-hydroxydopamine were incubated in 5 or 200 μmol·l-1 adrenaline at 37°C. Catechol-O-methyltransferase and monoamine oxidase were inhibited by U-0521 and pargyline, respectively, and the accumulation of adrenaline in trachealis smooth muscle cells was measured by fluorescence microphotometry. 2. The effects of anoxia, anoxia plus glucose deprivation, ouabain, reduced sodium (Na+) concentration, elevated potassium (K+) concentration and calcium (Ca2+) deprivation on adrenaline accumulation were examined in tissues incubated in adrenaline for 5 min. 3. Adrenaline accumulation in the trachealis smooth muscle cells was reduced by inhibition of oxidative metabolism (anoxia and glucose deprivation), by ouabain (100 μmol·l-1), by a reduction in Na+ concentration from 139 mmol·l-1 to 25 mmol·l-1, and by increases in K+ concentration, but not by omission of Ca2+. 4. These results from guinea-pig trachealis smooth muscle cells, in combination with those obtained by other workers on various organs and tissues, indicate that the extraneuronal accumulation of catecholamines requires energy derived from oxidative metabolism and is very sensitive to inhibition by increases in K+ concentration. However, there is marked variability in the effects of ouabain and of reduced Na+ or Ca2+ concentrations on the accumulation.
Keyword Adrenaline
Extraneuronal uptake
Fluorescence microphotometry
Ionic changes
Smooth muscle
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 23 Aug 2016, 13:47:17 EST by System User