The effect of chronic ethanol consumption on muscarinic receptors in rat brain

Pietzak E.R., Wilce P.A. and Shanley B.C. (1988) The effect of chronic ethanol consumption on muscarinic receptors in rat brain. Neurochemistry International, 12 4: 447-452. doi:10.1016/0197-0186(88)90027-7

Author Pietzak E.R.
Wilce P.A.
Shanley B.C.
Title The effect of chronic ethanol consumption on muscarinic receptors in rat brain
Journal name Neurochemistry International   Check publisher's open access policy
ISSN 0197-0186
Publication date 1988-01-01
Sub-type Article (original research)
DOI 10.1016/0197-0186(88)90027-7
Volume 12
Issue 4
Start page 447
End page 452
Total pages 6
Language eng
Subject 1307 Cell Biology
1312 Molecular Biology
2804 Cellular and Molecular Neuroscience
Abstract Ethanol (15% v/v) was administered in the drinking water to male Wistar rats over period of 3 months. Binding properties of muscarinic receptors were studied in synaptosomes from selected brain areas using [3H]quinuclidinyl benzilate and its displacement by the selective antagonist, pirenzepine and the agonist, carbachol. Dissociation constants (Kd) of all three ligands in the cerebral cortex, hippocampus and striatum of ethanol-treated groups did not differ from those in controls. Density of [3H]quinuclidinyl benzilate binding sites in the cortex of ethanol-treated animals was approx. 50% higher than in controls (2.06 ± 0.2 and 1.32 ± 0.2 pmol/mg of protein respectively, mean ± SD, n = 6, P < 0.001). This was largely attributable to an increase in M1 binding sites as shown by pirenzepine displacement studies. In the hippocampus and striatum binding capacity of muscarinic receptors was not affected by ethanol treatment. Synthesis of acetylcholine in cerebral cortex prisms from ethanol-treated animals was not inhibited under resting conditions, but stimulation of synthesis by high K+ concentration was significantly altenuated by comparison with controls. These results suggest that chronic ethanol consumption induces changes in cholinergic neurotransmission in selected brain areas.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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