A 31P-NMR study of phosphate transport and compartmentation in Candida utilis

Bourne R.M. (1990) A 31P-NMR study of phosphate transport and compartmentation in Candida utilis. BBA - Molecular Cell Research, 1055 1: 1-9. doi:10.1016/0167-4889(90)90084-Q


Author Bourne R.M.
Title A 31P-NMR study of phosphate transport and compartmentation in Candida utilis
Journal name BBA - Molecular Cell Research   Check publisher's open access policy
ISSN 0167-4889
Publication date 1990-10-15
Sub-type Article (original research)
DOI 10.1016/0167-4889(90)90084-Q
Open Access Status
Volume 1055
Issue 1
Start page 1
End page 9
Total pages 9
Subject 1304 Biophysics
1307 Cell Biology
1312 Molecular Biology
Abstract The recovery of Candida utilis from phosphate starvation was studied using 31P-NMR. The phosphate analogue methylphosphonate was found to be a useful indicator of cytosol pH. Added orthophosphate was rapidly accumulated by the cells and stored mainly In a stable pool of polyphosphate of mean chain-length at least 200 units. Observed pH changes in the medium and cytosol during uptake of orthophosphate and methylphosphonate are consistent with the transport of these compounds across the plasma membrane by a proton/phosphate symport. However, transport of phosphate across the vacuole membrane occurs by a mechanism for which methylphosphonate is not a substrate. In the cytosol pH changes are strongly correlated with changes in orthophosphate concentration, however, this is not the case in the vacuole.
Keyword (C. utilis)
NMR, 31P-
Phosphate starvation
Phosphate transport
Polyphosphate synthesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 23 Aug 2016, 13:40:03 EST by System User