In vitro interactions of phenformin with serum lipoproteins and consequences thereof

Cham B.E. (1977) In vitro interactions of phenformin with serum lipoproteins and consequences thereof. Chemico-Biological Interactions, 17 2: 193-201. doi:10.1016/0009-2797(77)90084-9


Author Cham B.E.
Title In vitro interactions of phenformin with serum lipoproteins and consequences thereof
Journal name Chemico-Biological Interactions   Check publisher's open access policy
ISSN 0009-2797
Publication date 1977-01-01
Sub-type Article (original research)
DOI 10.1016/0009-2797(77)90084-9
Volume 17
Issue 2
Start page 193
End page 201
Total pages 9
Subject 3005 Toxicology
Abstract Turbidity developed when phenformin was added to human serum; this turbidity increased in a sigmoidal fashion with rising concentrations of phenformin (5-50 nmole/1). Centrifugation produced clearing of the solution, with collection of particulate matter on the surface of the sera. Extraction of control, and phenformin-treated sera with petroleum ether for 15 min. revealed that cholesterol and triglyceride were responsible for the turbidity. Different sera produced different turbidities with a given concentration of phenformin. No significant simple correlation existed between turbidity and serum cholesterol and/or triglyceride levels. The turbidities, produced by the addition of a constant concentration of phenformin to a series of diluted serum samples, were linearly related to the amount of serum present. The turbidities acquired by purified very-low density (VLDL), low-density (LDL), and high-density lipoprotein (HDL) fractions with phenformin were additive, and the turbidity of phenformin-treated serum was accounted for by these lipoprotein fractions. Serum free of lipoproteins did not become turbid when exposed to phenformin. Phenformin added to serum which had previously been delipidated, failed to produce turbidity. The turbidity produced by phenformin was reversible, because it could easily be cleared by dialysis. No significant differences in quantitative immunochemical reactivities were observed when control serum was compared with the subnatant of phenformin-treated serum, as determined by single radial immunodiffusion with LDL antibodies. These in vitro observations may be related to the in vivo hypolipidemic action of phenformin on hyperlipidemic subjects.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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