A case of mistaken identity: CD11c-eYFP+ cells in the normal mouse brain parenchyma and neural retina display the phenotype of microglia, not dendritic cells

Dando, Samantha J., Naranjo Golborne, Cecilia, Chinnery, Holly R., Ruitenberg, Marc J. and McMenamin, Paul G. (2016) A case of mistaken identity: CD11c-eYFP+ cells in the normal mouse brain parenchyma and neural retina display the phenotype of microglia, not dendritic cells. Glia, 64 8: 1331-1349. doi:10.1002/glia.23005


Author Dando, Samantha J.
Naranjo Golborne, Cecilia
Chinnery, Holly R.
Ruitenberg, Marc J.
McMenamin, Paul G.
Title A case of mistaken identity: CD11c-eYFP+ cells in the normal mouse brain parenchyma and neural retina display the phenotype of microglia, not dendritic cells
Formatted title
A case of mistaken identity: CD11c-eYFP+ cells in the normal mouse brain parenchyma and neural retina display the phenotype of microglia, not dendritic cells
Journal name Glia   Check publisher's open access policy
ISSN 1098-1136
0894-1491
Publication date 2016-08-01
Sub-type Article (original research)
DOI 10.1002/glia.23005
Open Access Status Not yet assessed
Volume 64
Issue 8
Start page 1331
End page 1349
Total pages 19
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Formatted abstract
Under steady-state conditions the central nervous system (CNS) is traditionally thought to be devoid of antigen presenting cells; however, putative dendritic cells (DCs) expressing enhanced yellow fluorescent protein (eYFP) are present in the retina and brain parenchyma of CD11c-eYFP mice. We previously showed that these mice carry the Crb1rd8 mutation, which causes retinal dystrophic lesions; therefore we hypothesized that the presence of CD11c-eYFP+ cells within the CNS may be due to pathology associated with the Crb1rd8 mutation. We generated CD11c-eYFP Crb1wt/wt mice and compared the distribution and immunophenotype of CD11c-eYFP+ cells in CD11c-eYFP mice with and without the Crb1rd8 mutation. The number and distribution of CD11c-eYFP+ cells in the CNS was similar between CD11c-eYFP Crb1wt/wt and CD11c-eYFP Crb1rd8/rd8 mice. CD11c-eYFP+ cells were distributed throughout the inner retina, and clustered in brain regions that receive input from the external environment or lack a blood-brain barrier. CD11c-eYFP+ cells within the retina and cerebral cortex of CD11c-eYFP Crb1wt/wt mice expressed CD11b, F4/80, CD115 and Iba-1, but not DC or antigen presentation markers, whereas CD11c-eYFP+ cells within the choroid plexus and pia mater expressed CD11c, I-A/I-E, CD80, CD86, CD103, DEC205, CD8α and CD135. The immunophenotype of CD11c-eYFP+ cells and microglia within the CNS was similar between CD11c-eYFP Crb1wt/wt and CD11c-eYFP Crb1rd8/rd8 mice; however, CD11c and I-A/I-E expression was significantly increased in CD11c-eYFP Crb1rd8/rd8 mice. This study demonstrates that the overwhelming majority of CNS CD11c-eYFP+ cells do not display the phenotype of DCs or their precursors and are most likely a subpopulation of microglia.
Keyword Microglia
Central nervous system
Crumbs 1
Retinal dystrophic lesions
Antigen presenting cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
School of Biomedical Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
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Created: Fri, 19 Aug 2016, 02:03:23 EST by Susan Day on behalf of Queensland Brain Institute