Measuring survival of adherent cells with the colony-forming assay

Crowley, Lisa C., Christensen, Melinda E. and Waterhouse, Nigel J. (2016) Measuring survival of adherent cells with the colony-forming assay. Cold Spring Harbor Protocols, 2016 8: 721-724. doi:10.1101/pdb.prot087171


Author Crowley, Lisa C.
Christensen, Melinda E.
Waterhouse, Nigel J.
Title Measuring survival of adherent cells with the colony-forming assay
Journal name Cold Spring Harbor Protocols   Check publisher's open access policy
ISSN 1940-3402
1559-6095
Publication date 2016-08-01
Year available 2016
Sub-type Article (original research)
DOI 10.1101/pdb.prot087171
Open Access Status Not yet assessed
Volume 2016
Issue 8
Start page 721
End page 724
Total pages 4
Place of publication Cold Spring Harbor, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provide an indirect measurement of cell death. This can be achieved using the colony-forming assay described here. This protocol specifically applies to measurement of HeLa cells but can be used for most adherent cell lines with limited motility.
Keyword Cell death
Colony-forming assay
HeLa cells
Adherent cells
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes All Authors Mater Research-UQ affiliated

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
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Admin Only - School of Medicine
School of Medicine Publications
 
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Created: Thu, 18 Aug 2016, 20:48:51 EST by Julia McCabe on behalf of Learning and Research Services (UQ Library)