Measurement of cytochrome f in thylakoids of higher plants using quantitative gel electrophoresis

Holloway P.J., Maclean D.J. and Scott K.J. (1987) Measurement of cytochrome f in thylakoids of higher plants using quantitative gel electrophoresis. Analytical Biochemistry, 164 1: 31-34. doi:10.1016/0003-2697(87)90362-9


Author Holloway P.J.
Maclean D.J.
Scott K.J.
Title Measurement of cytochrome f in thylakoids of higher plants using quantitative gel electrophoresis
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1987-01-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(87)90362-9
Volume 164
Issue 1
Start page 31
End page 34
Total pages 4
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A quantitative method was developed to estimate the concentration of cytochrome (cyt) f in isolated thylakoids, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by staining with a heme-specific reagent containing 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide. This densitometric technique was at least as sensitive as difference spectroscopy. Analysis of thylakoid preparations by densitometry of stained bands using cyt c as standard gave molar ratios of cyt/chlorophyll which were identical to ratios obtained by difference spectroscopy. Densitometric assays demonstrated that the molar ratio of cyt f/chlorophyll decreased during leaf aging in seven higher plants; however, there was a marked difference in the rate at which cyt f was lost from the leaves of different species.
Keyword cytochrome f
gel electrophoresis
heme-dependent peroxidase
photosynthesis
tetramethylbenzidine
thylakoids
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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