Experience in scale-up of homogeneous perfusion culture for hybridomas

Greenfield P.F., Guillaume J.-M., Randerson D.H. and Smith C.G. (1991) Experience in scale-up of homogeneous perfusion culture for hybridomas. Bioprocess Engineering, 6 5: 213-219. doi:10.1007/BF00369714

Author Greenfield P.F.
Guillaume J.-M.
Randerson D.H.
Smith C.G.
Title Experience in scale-up of homogeneous perfusion culture for hybridomas
Journal name Bioprocess Engineering   Check publisher's open access policy
ISSN 0178-515X
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1007/BF00369714
Volume 6
Issue 5
Start page 213
End page 219
Total pages 7
Publisher Springer-Verlag
Subject 2402 Applied Microbiology and Biotechnology
1305 Biotechnology
Abstract A perfusion system for production of monoclonal antibodies was developed using an externally-mounted, hollow-fibre cartridge. The experimental apparatus was operated for 420 h and demonstrated increased steady-state viable cell concentration with increase in perfusion rate. Antibody titres were up to three times those measured for batch cultures and specific antibody productivity was doubled. The procedure was successfully scaled to a 10 dm3 system which produced antibody under conditions of Good Manufacturing Practice (GMP). A calculation of productivity between the scaled perfusion system and 260 dm3 batch cultures resulted in comparable antibody production, whereas the perfusion allowed a halving in medium utilisation. Reactivity assays conducted on the purified antibody from both batch and perfusion cultures showed no evidence of proteolysis or altered antibody activity in the final perfusion product. This study provides additional support for the use of homogeneous perfusion cultures in production of monoclonal antibodies under GMP conditions.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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