Histochemical activity of acid phosphatase in rat liver after perfusion fixation

Khan M.A. and Angus B.M. (1980) Histochemical activity of acid phosphatase in rat liver after perfusion fixation. Cells Tissues Organs, 106 3: 327-329. doi:10.1159/000145196


Author Khan M.A.
Angus B.M.
Title Histochemical activity of acid phosphatase in rat liver after perfusion fixation
Journal name Cells Tissues Organs   Check publisher's open access policy
ISSN 1422-6421
Publication date 1980-01-01
Sub-type Article (original research)
DOI 10.1159/000145196
Volume 106
Issue 3
Start page 327
End page 329
Total pages 3
Subject 2702 Anatomy
2722 Histology
Abstract Acid phosphatase activity was studied histochemically in rat livers after perfusion fixation. The three different perfusates used were: (1) 2% of paraformaldehyde; (2) 1.25% of glutaraldehyde, and (3) 1.25% of each of paraformaldehyde and glutaraldehyde. Unfixed fresh frozen sections showed high enzymatic activity in both centrilobular (CL) and periportal (PP) areas. A similar localization pattern resulted after perfusate 1 indicating a lack of reduction in the enzymatic activity in either of the two areas. After perfusate 2, PP areas remained intensely stained while slight reduction of the enzymatic activity was exhibited by the CL regions. Perfusion (3) resulted in higher activity in PP areas but maximal inhibition in the CL region. The inhibition of acid phosphatase activity in the CL areas correlates with regional differences of Kupffer cell population as well as the existence of metabolic heterogeneity between the two hepatic regions.
Keyword Acid phosphatase
Histochemistry
Perfusion fixation
Rat liver
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 16 Aug 2016, 13:38:48 EST by System User