Properties of a purple phosphatase from red kidney bean: a zinc-iron metalloenzyme

Beck J.L., McConachie L.A., Summors A.C., Arnold W.N., De Jersey J. and Zerner B. (1986) Properties of a purple phosphatase from red kidney bean: a zinc-iron metalloenzyme. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 869 1: 61-68. doi:10.1016/0167-4838(86)90310-9


Author Beck J.L.
McConachie L.A.
Summors A.C.
Arnold W.N.
De Jersey J.
Zerner B.
Title Properties of a purple phosphatase from red kidney bean: a zinc-iron metalloenzyme
Journal name Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
ISSN 0167-4838
Publication date 1986-01-17
Sub-type Article (original research)
DOI 10.1016/0167-4838(86)90310-9
Open Access Status Not yet assessed
Volume 869
Issue 1
Start page 61
End page 68
Total pages 8
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
1315 Structural Biology
Abstract A purple phosphatase from the red kidney bean Phaseolus vulgaris has been purified to homogeneity and characterized. It resembles sweet potato purple acid phosphatase in being a dimer of approx. 130 kDa and in its amino acid composition and visible absorption spectrum. The red kidney bean enzyme contains one atom of iron and one atom of zinc per subunit, whereas the sweet potato enzyme is reported to contain manganese. The visible absorption spectrum shows a λmax at 560 nm with ε{lunate}560 (per iron) = 3360 M- · cm-1, and is destroyed by dithionite treatment. Red kidney bean phosphatase shows a marked preference for ATP as substrate over p-nitrophenyl phosphate and ADP. Stable esters such as AMP and β-glycerophosphate are very poor substrates. The enzyme is compared with other purple phosphatases from plant and animal sources.
Keyword (Red kidney bean)
ATPase
Fe
Metal enzyme
Purple phosphatase
Zn
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 126 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 16 Aug 2016, 12:05:18 EST by System User