The effect of hypophysectomy on testicular hydrolases, lactate dehydrogenase, and spermatogenesis in the rat

Elkington J.S.H., Blackshaw A.W. and De Jong B. (1973) The effect of hypophysectomy on testicular hydrolases, lactate dehydrogenase, and spermatogenesis in the rat. Australian Journal of Biological Sciences, 26 2: 491-503. doi:10.1071/BI9730491


Author Elkington J.S.H.
Blackshaw A.W.
De Jong B.
Title The effect of hypophysectomy on testicular hydrolases, lactate dehydrogenase, and spermatogenesis in the rat
Journal name Australian Journal of Biological Sciences   Check publisher's open access policy
ISSN 0004-9417
Publication date 1973-01-01
Sub-type Article (original research)
DOI 10.1071/BI9730491
Open Access Status Not yet assessed
Volume 26
Issue 2
Start page 491
End page 503
Total pages 13
Subject 1103 Clinical Sciences
1309 Developmental Biology
1311 Genetics
1305 Biotechnology
1312 Molecular Biology
1310 Endocrinology
2743 Reproductive Medicine
2700 Medicine
Abstract Hypophysectomy of the adult male rat depressed testis and seminal vesicle weight between 4 and 8 days after hypophysectomy. The activity of soluble acid proteinase (E.C. 3.4.4.23) and, B-glucuronidase (E.C. 3.2.1.31) increased between 8 and 16 days after the operation, while that for acid phosphatase (E.C. 3.1.3.2) increased until 16 days after hypophysectomy and then decreased to the end of the experiment. There was an increase in the total acid proteinase and, β-glucuronidase activity per gram testis between 8 and 16 days after hypophysectomy; there was no change in the total activity of acid phosphatase per gram testis until 16-32 days after treatment, when there was a decrease. After hypophysectomy there was a decrease in acid phosphatase activity per testis after 16 days, and acid proteinase activity per testis after 8 days, while there was an increase in both free and total, β-glucuronidase activity per testis after 16 days. A decrease in lactate dehydrogenase isoenzyme (LDH-X) activity in the testis occurred between 8 and 16 days after the operation. Histochemical evidence showed an increase in acid phosphatase activity in the seminiferous tubules 4-8 days after hypophysectomy, which coincided with a decrease in the yield of round spermatids from late primary sperma tocytes and of long spermatids from round spermatids. It is concluded that lysosomal enzymes of Sertoli cell origin are important in testicular degeneration and, probably, in normal spermatogenesis. Acid phosphatase and acid proteinase are found mainly in spermatocytes and spermatids, while, β-glucuronidase is found mainly in the Sertoli cells and spermatogonia. LDH-X occurs in the seminiferous tubular epithelium associated with late primary spermatocytes and spermatids.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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