Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae

Hardjito L., Greenfield P.F. and Lee P.L. (1993) Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae. Enzyme and Microbial Technology, 15 2: 120-126. doi:10.1016/0141-0229(93)90035-Z


Author Hardjito L.
Greenfield P.F.
Lee P.L.
Title Recombinant protein production via fed-batch culture of the yeast Saccharomyces cerevisiae
Journal name Enzyme and Microbial Technology   Check publisher's open access policy
ISSN 0141-0229
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1016/0141-0229(93)90035-Z
Volume 15
Issue 2
Start page 120
End page 126
Total pages 7
Subject 1303 Specialist Studies in Education
1305 Biotechnology
2402 Applied Microbiology and Biotechnology
Abstract Protein production with the recombinant yeast Saccharomyces cerevisiae in fed-batch culture is investigated in this work using β-galactosidase as a model protein. Segregational instability was negligible during the observed culture periods. The final volumetric productivity, as determined by both cell concentration and gene expression, was strongly affected by the time course of the glucose levels in the bioreactor. It was found that an average glucose feed rate of 1.31 g glucose h-1 resulted in both the maximum β-galactosidase production rate of 831-950 units ml-1 h-1 and the maximum cell production rate of 0.520-0.524 mg ml-1 h-1.
Keyword fed-batch glucose concentration
recombinant protein
Saccharomyces cerevisiae
β-galactosidase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 25 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 16 Aug 2016, 11:40:05 EST by System User