Inhibition of sterol and DNA synthesis in peripheral blood lymphocytes by AY9944

Kay G. and Wilce P.A. (1983) Inhibition of sterol and DNA synthesis in peripheral blood lymphocytes by AY9944. Biochemical and Biophysical Research Communications, 110 1: 82-87. doi:10.1016/0006-291X(83)91263-9


Author Kay G.
Wilce P.A.
Title Inhibition of sterol and DNA synthesis in peripheral blood lymphocytes by AY9944
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1983-01-14
Sub-type Article (original research)
DOI 10.1016/0006-291X(83)91263-9
Open Access Status Not yet assessed
Volume 110
Issue 1
Start page 82
End page 87
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract The effect of the inhibitor of cholesterol synthesis AY9944 on DNA synthesis was tested in phytohemagglutinin stimulated lymphocytes incubated in delipidated serum. AY9944 caused a decrease in cholesterol synthesis from [14C] acetate and an accummulation of [14C] 7-dehydrocholesterol. This inhibition of cholesterol synthesis resulted in an inhibition of DNA synthesis in a dose related manner. Inhibition could be partly reversed by added lipoprotein. Inhibition of DNA synthesis occurred if AY9944 was added at the early stages of blast transformation but not when the response was well established. These findings suggest that some endogenous cholesterol synthesis may be required for the complete phytohemagglutinin stimulation of lymphocytes.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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