Separation of radioiodinated angiotensins by chromatofocusing in minicolumns

Sernia C. (1984) Separation of radioiodinated angiotensins by chromatofocusing in minicolumns. Analytical Biochemistry, 138 2: 303-308. doi:10.1016/0003-2697(84)90812-1


Author Sernia C.
Title Separation of radioiodinated angiotensins by chromatofocusing in minicolumns
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1984-05-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(84)90812-1
Volume 138
Issue 2
Start page 303
End page 308
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Angiotensins I, II, and III (AI, AII, AIII) and Saralasin (Sar1-Ala8-AII) were labeled with 125I and separated from the nonlabeled forms on minicolumns (a Pasteur pipet) of chromatofocusing medium. At low ionic strength, 125I-labeled angiotensins could be eluted with Polybuffer or a piperazine-histidine buffer at their approximate isoelectric points, while nonlabeled angiotensins remained adsorbed to the column and required 1 mol · liter-1 NaCl for elution. The 125I-labeled angiotensins prepared by this method were bound by antibodies (AI) and adrenal receptors (AII, Saralasin) to an extent similar to angiotensins prepared by DEAE-Sephadex A-25 chromatography. This new method of preparing radioiodinated angiotensins is rapid (15 min), inexpensive, and requires no fraction-collecting equipment.
Keyword angiotensins
chromatofocusing
chromatography
hormone
peptides
radioiodine labeling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 16 Aug 2016, 11:22:25 EST by System User