16S-rDNA analysis of Spirochaeta thermophila: Its phylogenetic position and implications for the systematics of the order Spirochaetales

Rainey F.A., Dorsch M., Morgan H.W. and Stackebrandt E. (1992) 16S-rDNA analysis of Spirochaeta thermophila: Its phylogenetic position and implications for the systematics of the order Spirochaetales. Systematic and Applied Microbiology, 15 2: 197-202. doi:10.1016/S0723-2020(11)80092-5


Author Rainey F.A.
Dorsch M.
Morgan H.W.
Stackebrandt E.
Title 16S-rDNA analysis of Spirochaeta thermophila: Its phylogenetic position and implications for the systematics of the order Spirochaetales
Journal name Systematic and Applied Microbiology   Check publisher's open access policy
ISSN 0723-2020
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1016/S0723-2020(11)80092-5
Open Access Status Not yet assessed
Volume 15
Issue 2
Start page 197
End page 202
Total pages 6
Language eng
Subject 2402 Applied Microbiology and Biotechnology
Abstract The 16S rRNA gene of Spirochaeta thermophila DSM 6578 was amplifed by the polymerase chain reaction and analysis of a 1333 nucleotide long stretch performed. The sequence was aligned to the homologous region of 13 representatives from six spirochete genera, and the phylogenetic position of S. thermophila determined. This species constitutes a deep-branching member of a cluster that is defined by representatives of Spirochaeta and Treponema and by Borellia burgdorferi. The genera Serpulina, Leptonema and Leptospira are more ancient representatives of the spirochete line of descent. The branching pattern confirms earlier results of phylogenetic studies which showed the genus Spirochaeta to be heterogeneous, with S. zuelzerae and S. stenostrepta displaying a higher degree of relatedness to treponemas than to the main (authentic) Spirochaeta group.
Keyword 16S rRNA gene amplification
PCR
phylogeny
Spirochaeta thermophila
spirochetes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 16 Aug 2016, 11:14:59 EST by System User