The establishment of human T cell lines reactive with specific periodontal bacteria.

Ishii T., Mahanonda R. and Seymour G.J. (1992) The establishment of human T cell lines reactive with specific periodontal bacteria.. Oral microbiology and immunology, 7 4: 225-229. doi:10.1111/j.1399-302X.1992.tb00029.x


Author Ishii T.
Mahanonda R.
Seymour G.J.
Title The establishment of human T cell lines reactive with specific periodontal bacteria.
Journal name Oral microbiology and immunology   Check publisher's open access policy
ISSN 0902-0055
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1111/j.1399-302X.1992.tb00029.x
Open Access Status Not yet assessed
Volume 7
Issue 4
Start page 225
End page 229
Total pages 5
Language eng
Subject 2403 Immunology
2726 Microbiology (medical)
3500 Dentistry
Abstract Human T cell lines (TCLs) were obtained by stimulation of peripheral blood mononuclear cells (PBMCs) with the 2 periodontopathic bacteria, Porphyromonas gingivalis FDC‐381 and Fusobacterium nucleatum FDC‐263. After the first round of stimulation and rest, the cells responded specifically to the bacteria originally used to establish each line. Throughout the culture period, the responsiveness of each of the TCLs to their specific bacteria increased. Phenotypic analysis of the TCLs revealed heterogeneity of cell types. In both TCLs approximately 80% of the cells were T cells, all of which bore the αβ T cell receptor. The P. gingivalis‐reactive TCL (PG‐TCL) showed approximately equal proportions of CD4+ and CD8+ cells, whereas the P. nucleatum‐reactive TCL (FN‐TCL) was predominantly CD4+. The expression of CD25, HLA‐DR, CD45RA and CD29 on these CD4+ cells varied throughout the culture period of 45 days. These results demonstrate that it is possible to establish long‐term T cell lines reacting to specific periodontopathic bacteria. Copyright
Keyword periodontopathic bacteria
T cell
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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