Localization of a brain sulfotransferase, SULT4A1, in the human and rat brain: An immunohistochemical study

Liyou, N. E., Buller, K. M., Tresillian, M. J., Elvin, C. M., Scott, H. L., Dodd, P. R., Tannenberg, A. E. G. and McManus, M. E. (2003) Localization of a brain sulfotransferase, SULT4A1, in the human and rat brain: An immunohistochemical study. Journal of Histochemistry and Cytochemistry, 51 12: 1655-1664. doi:10.1177/002215540305101209


Author Liyou, N. E.
Buller, K. M.
Tresillian, M. J.
Elvin, C. M.
Scott, H. L.
Dodd, P. R.
Tannenberg, A. E. G.
McManus, M. E.
Title Localization of a brain sulfotransferase, SULT4A1, in the human and rat brain: An immunohistochemical study
Journal name Journal of Histochemistry and Cytochemistry   Check publisher's open access policy
ISSN 0022-1554
Publication date 2003-12-01
Year available 2003
Sub-type Article (original research)
DOI 10.1177/002215540305101209
Open Access Status Not yet assessed
Volume 51
Issue 12
Start page 1655
End page 1664
Total pages 10
Place of publication Seattle, United States
Publisher Histochemical Society
Language eng
Subject 2702 Anatomy
2722 Histology
Abstract Cytosolic sulfotransferases are believed to play a role in the neuromodulation of certain neurotransmitters and drugs. To date, four cytosolic sulfotransferases have been shown to be expressed in human brain. Recently, a novel human brain sulfotransferase has been identified and characterized, although its role and localization in the brain are unknown. Here we present the first immunohistochemical (IHC) localization of SULT4A1 in human brain using an affinity-purified polyclonal antibody raised against recombinant human SULT4A1. These results are supported and supplemented by the IHC localization of SULT4A1 in rat brain. In both human and rat brains, strong reactivity was found in several brain regions, including cerebral cortex, cerebellum, pituitary, and brainstem. Specific signal was entirely absent on sections for which preimmune serum from the corresponding animal, processed in the same way as the postimmune serum, was used in the primary screen. The findings from this study may assist in determining the physiological role of this SULT isoform.
Keyword Cell Biology
Immunohistochemistry
Sulfotransferase
Human Brain
Rat Brain
Neuron
Localization
Tick Boophilus-microplus
Hydroxysteroid Sulfotransferase
Phenol Sulfotransferase
Crystal-structure
5'-nucleotidase
Metabolism
Expression
Dopamine
Cloning
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Mon, 13 Aug 2007, 23:57:48 EST