Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy

Wang, Haolu, Liang, Xiaowen, Gravot, Germain, Thorling, Camilla A., Crawford, Darrell H. G., Xu, Zhi Ping, Liu, Xin and Roberts, Michael S. (2016) Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy. Journal of Biophotonics, 10 1: 46-60. doi:10.1002/jbio.201600083


Author Wang, Haolu
Liang, Xiaowen
Gravot, Germain
Thorling, Camilla A.
Crawford, Darrell H. G.
Xu, Zhi Ping
Liu, Xin
Roberts, Michael S.
Title Visualizing liver anatomy, physiology and pharmacology using multiphoton microscopy
Journal name Journal of Biophotonics   Check publisher's open access policy
ISSN 1864-063X
1864-0648
Publication date 2016-06-17
Year available 2017
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1002/jbio.201600083
Open Access Status Not yet assessed
Volume 10
Issue 1
Start page 46
End page 60
Total pages 15
Place of publication Weinheim, Germany
Publisher Wiley
Language eng
Subject 1600 Chemistry
2500 Materials Science
1300 Biochemistry, Genetics and Molecular Biology
2200 Engineering
3100 Physics and Astronomy
Abstract Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.
Formatted abstract
Multiphoton microscopy (MPM) has become increasingly popular and widely used in both basic and clinical liver studies over the past few years. This technology provides insights into deep live tissues with less photobleaching and phototoxicity, which helps us to better understand the cellular morphology, microenvironment, immune responses and spatiotemporal dynamics of drugs and therapeutic cells in the healthy and diseased liver. This review summarizes the principles, opportunities, applications and limitations of MPM in hepatology. A key emphasis is on the use of fluorescence lifetime imaging (FLIM) to add additional quantification and specificity to the detection of endogenous fluorescent species in the liver as well as exogenous molecules and nanoparticles that are applied to the liver in vivo. We anticipate that in the near future MPM-FLIM will advance our understanding of the cellular and molecular mechanisms of liver diseases, and will be evaluated from bench to bedside, leading to real-time histology of human liver diseases.
Keyword Multiphoton microscopy
Fluorescence lifetime imaging
Liver
Morphology
Function
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID APP1049979
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: HERDC Pre-Audit
Admin Only - School of Medicine
School of Medicine Publications
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 1 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 1 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Thu, 28 Jul 2016, 23:55:17 EST by Camilla Thompson on behalf of Medicine - Princess Alexandra Hospital