A low-molecular-weight acid phosphatase which contains iron

Campbell H.D. and Zerner B. (1973) A low-molecular-weight acid phosphatase which contains iron. Biochemical and Biophysical Research Communications, 54 4: 1498-1503. doi:10.1016/0006-291X(73)91155-8

Author Campbell H.D.
Zerner B.
Title A low-molecular-weight acid phosphatase which contains iron
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1973-10-15
Sub-type Article (original research)
DOI 10.1016/0006-291X(73)91155-8
Open Access Status Not yet assessed
Volume 54
Issue 4
Start page 1498
End page 1503
Total pages 6
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A simple, large scale purification procedure for the violet "phosphoprotein phosphatase" from beef spleen [Glomset, J. and Porath, J., Biochim. Biophys. Acta, 39, 1 (1960)] has been devised. The procedure involves a 24-hr extraction of a tissue homogenate at pH 3, ammonium sulfate fractionation, CM-cellulose chromatography, and Sephadex G-75 chromatography. The highly purified enzyme shows a constant ratio of both enzymatic activity and absorbance at 550 nm to iron concentration. The data strongly indicate that the enzyme contains iron, and moreover, support a 1:1 molar ratio of iron to enzyme.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Created: Tue, 26 Jul 2016, 14:48:23 EST by System User