Modulation of pteroylpolyglutamate concentratin and length in response to altered folate nutrition in a comprehensive range of rat tissues

Ward G.J. and Nixon P.F. (1990) Modulation of pteroylpolyglutamate concentratin and length in response to altered folate nutrition in a comprehensive range of rat tissues. Journal of Nutrition, 120 5: 476-484.

Author Ward G.J.
Nixon P.F.
Title Modulation of pteroylpolyglutamate concentratin and length in response to altered folate nutrition in a comprehensive range of rat tissues
Journal name Journal of Nutrition   Check publisher's open access policy
ISSN 0022-3166
Publication date 1990-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 120
Issue 5
Start page 476
End page 484
Total pages 9
Language eng
Subject 1106 Human Movement and Sports Science
2701 Medicine (miscellaneous)
Abstract For a range of rat tissue extracts, the concentrations of total folates and of short-chain pteroylpolyglutamates were assayed by Lactobacillus casei with and without conjugase treatment, respectively, and the concentration and chain length of HPte-Gln(n) and 5,10-CH-HPteGln(n) together were assayed after binding to thymidylate synthase and tritiated fluorodeoxyuridylate. For rats fed a nonpurified diet and consuming 26 μg of folic acid daily, the respective concentrations of these total folates, short-chain folates and thymidylate synthase bindable folates were, in nmol/g, 10.2, 2.5 and 3.5 in liver, 3.9, 1.8 and 2.0 in kidney, 4.2, 1.2 and 1.0 in bone marrow, 2.3, 0.6 and 0.2 in adrenal, 2.1, 0.3 and 0.5 in spleen, 2.1, 0.9 and 0.8 in jejunal smooth muscle, 1.2, 0.9 and 0.2 in jejunal mucosa, 1.0, 0.3 and 0.6 in testis, 0.7, 0.1 and 0.2 in heart, 0.3, 0.1 and 0.1 in skeletal muscle, 0.5, 0.1 and 0.2 in brain and 0.7, 0.002 and 0 in erythrocytes. The predominant pteroylpolyglutamate chain length was 6 residues in all tissues except kidney, jejunal mucosa, skeletal muscle and brain, in which the value was 5 residues. A folate-deficient diet (30 ng/d) fed for 3 wk resulted in a depression in the total folate concentration of all tissues (except brain); the depression was generally greater for short-chain than for long-chain folates and was accompanied by a lengthening of the pteroylpolyglutamate chain. Opposite results followed folate excess of 4 to 5.4 mg/d. The fractional change in the folate concentration of the individual tissues, following perturbation of dietary folate, did not vary greatly among tissues. These results are interpreted as evidence that folylpolyglutamate synthetase operates in most tissues to increase chain length in periods of folate deficiency and that there is no particular tissue whose pool of folates constitutes a metabolically inactive store.
Keyword folate deficiency
folate excess
pteroylpolyglutamates
rats
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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