Male infertility in mice lacking the store-operated Ca(2+) channel Orai1

Davis, Felicity M., Goulding, Eugenia H., D'Agostin, Diane M., Janardhan, Kyathanahalli S., Cummings, Connie A., Bird, Gary S., Eddy, Edward M. and Putney, James W. (2016) Male infertility in mice lacking the store-operated Ca(2+) channel Orai1. Cell Calcium, 59 4: 189-197. doi:10.1016/j.ceca.2016.02.007

Author Davis, Felicity M.
Goulding, Eugenia H.
D'Agostin, Diane M.
Janardhan, Kyathanahalli S.
Cummings, Connie A.
Bird, Gary S.
Eddy, Edward M.
Putney, James W.
Title Male infertility in mice lacking the store-operated Ca(2+) channel Orai1
Formatted title
Male infertility in mice lacking the store-operated Ca(2+) channel Orai1
Journal name Cell Calcium   Check publisher's open access policy
ISSN 0143-4160
Publication date 2016-04-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.ceca.2016.02.007
Open Access Status Not Open Access
Volume 59
Issue 4
Start page 189
End page 197
Total pages 9
Place of publication London, United Kingdom
Publisher Churchill Livingstone
Language eng
Formatted abstract
Store-operated calcium entry (SOCE) is an important Ca2+ influx pathway in somatic cells. In addition to maintaining endoplasmic reticulum (ER) Ca2+ stores, Ca2+ entry through store-operated channels regulates essential signaling pathways in numerous cell types. Patients with mutations in the store-operated channel subunit ORAI1 exhibit defects in store-operated Ca2+ influx, along with severe immunodeficiency, congenital myopathy and ectodermal dysplasia. However, little is known about the functional role of ORAI1 in germ cells and reproductive function in mice, or in men, since men with loss-of-function or null mutations in ORAI1 rarely survive to reproductive age. In this study, we investigated the role of ORAI1 in male reproductive function. We reveal that Orai1−/− male mice are sterile and have severe defects in spermatogenesis, with prominent deficiencies in mid- to late-stage elongating spermatid development. These studies establish an essential in vivo role for store-operated ORAI1 channels in male reproductive function and identify these channels as potential non-steroidal regulators of male fertility.
Keyword Cell Biology
Cell Biology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Pharmacy Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 6 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 13 Jul 2016, 06:00:57 EST by Miss Felicity Davis on behalf of School of Pharmacy