Reaction of MDCK cells to crystals of monosodium urate monohydrate and uric acid

Emmerson B.T., Cross M., Osborne J.M. and Axelsen R.A. (1990) Reaction of MDCK cells to crystals of monosodium urate monohydrate and uric acid. Kidney International, 37 1: 36-43.

Author Emmerson B.T.
Cross M.
Osborne J.M.
Axelsen R.A.
Title Reaction of MDCK cells to crystals of monosodium urate monohydrate and uric acid
Journal name Kidney International   Check publisher's open access policy
ISSN 0085-2538
Publication date 1990-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 37
Issue 1
Start page 36
End page 43
Total pages 8
Publisher Blackwell Publishing Inc.
Language eng
Subject 2727 Nephrology
Abstract Madin-Darby canine kidney (MDCK) cells exhibit many of the characteristics of cells of the cortical collecting tubule. Since hypotheses concerning the development of gouty and uric acid nephropathy involve a reaction between such cells and crystals, either of monosodium urate monohydrate (MSUM) or uric acid, the reaction between MDCK cells in culture and the above crystals was studied, both morphologically and functionally. In monolayer cultures, reaction sites developed within four hours of exposure to urate crystals. These increased in number for up to 72 hours and subsided gradually after removal of the crystals. At these reaction sites, crystals were observed to have passed beneath the cell surface and could be demonstrated both within intra-cellular lysosomes as well as within the inter-cellular spaces. When the MDCK cells were maintained as single cells in suspension, phagocytosis of crystals by the majority of the cells could be observed, but the response was much more rapid than in monolayers. During the cell/crystal reaction, significant amounts of lysosomal enzymes and prostaglandin E were released and, to a less significant degree, cytosolic enzymes, presumably due to cell lysis. This enzyme release did not occur in MDCK cells grown in protein-free medium, and protein coating of the crystals was necessary for reactivity with cells. In this regard, coating with IgG or lysozyme was more effective than albumin. The reaction with uric acid crystals revealed a reactivity which was lesser in degree but qualitatively similar to that of urate crystals. These studies support the hypothesis that one of the earliest lesions in uric acid or gouty nephropathy occurs between intra-luminal crystals and the epithelial cells of the distal portion of the nephron, and that some of the subsequent renal damage is secondary to this reaction.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Created: Tue, 12 Jul 2016, 13:54:29 EST by System User