Observations on the post-mortem migration of nematode larvae and its role in tissue digestion techniques

Prociv P. (1989) Observations on the post-mortem migration of nematode larvae and its role in tissue digestion techniques. Journal of Helminthology, 63 4: 281-286. doi:10.1017/S0022149X00009159


Author Prociv P.
Title Observations on the post-mortem migration of nematode larvae and its role in tissue digestion techniques
Journal name Journal of Helminthology   Check publisher's open access policy
ISSN 1475-2697
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1017/S0022149X00009159
Volume 63
Issue 4
Start page 281
End page 286
Total pages 6
Subject 2405 Parasitology
1103 Clinical Sciences
Abstract The importance of larval viability in relation to the success of recovery techniques and to the interpretation of histological and migration studies was tested using mice infected with Toxocara pteropodis, T. canis and T. cati. Little difference was found in total numbers of viable larvae recovered from tissues incubated for 18 hours in 0.85% saline, 2% trypsin or 1% pepsin, but the rates of larval egress did vary. The recovery of dead larvae by digestion techniques was much lower. Larvae migrated in tissues undergoing fixation, which influenced histological findings. Larvae of T. canis did not undergo significant peritoneal migration in acutely-infected mice, but did penetrate their intestinal walls in large numbers within 4 hours of death. The implications of these findings in experimental studies are discussed.
Keyword Ascaris lumbricoides
digestion techniques
larval migration
mouse
nematode larvae
Toxocara canis
Toxocara cati
Toxocara pteropodis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Tue, 12 Jul 2016, 13:51:42 EST by System User