Synthesis of purines in human lymphoblast cells deficient in methylthioadenosine phosphorylase activity

Gordon R.B., Blackwell K. and Emmerson B.T. (1987) Synthesis of purines in human lymphoblast cells deficient in methylthioadenosine phosphorylase activity. BBA - Molecular Cell Research, 927 1: 1-7. doi:10.1016/0167-4889(87)90059-0


Author Gordon R.B.
Blackwell K.
Emmerson B.T.
Title Synthesis of purines in human lymphoblast cells deficient in methylthioadenosine phosphorylase activity
Journal name BBA - Molecular Cell Research   Check publisher's open access policy
ISSN 0167-4889
Publication date 1987-01-19
Sub-type Article (original research)
DOI 10.1016/0167-4889(87)90059-0
Open Access Status
Volume 927
Issue 1
Start page 1
End page 7
Total pages 7
Subject 1304 Biophysics
1307 Cell Biology
1312 Molecular Biology
Abstract Two human lymphoblastic cell lines, deficient in methylthioadenosine phosphorylase (MTAP) activity, were found to have increased rates of de novo purine synthesis. These MTAP- cell lines were K562, an undifferentiated leukemic line and CCRF-CEM, a leukemic line of T-cell origin. Another T-cell line, CCRF-HSB-2 was found to be deficient in activity. However, this line did not demonstrate elevated rates of purine synthesis. Purine metabolism in the above cell cultures was compared with MTAP+ human B-cell lines and two human T-cell lines (MOLT-3 and MOLT-4). In all the MTAP+ cell lines, the rate of de novo purine synthesis was inhibited by the presence of methylthioadenosine in the assay medium (10 μM concentration produced more than 90% inhibition). However, purine synthesis in the MTAP- cells was resistant to inhibition by methylthioadenosine. Adenine in the assay medium inhibited de novo purine synthesis in MTAP+ and MTAP- cells to a similar degree. This inhibition was dose dependent and was elicited by concentrations similar to those of methylthioadenosine. Growth of the cell lines in culture was not affected by either methylthioadenosine or adenine at the concentrations which produced inhibition of purine synthesis. These results suggest that purine synthesis in MTAP+ cells is inhibited by adenine formed from the phosphorolytic cleavage of methylthioadenosine by methylthioadenosine phosphorylase.
Keyword Adenine
Methylthioadenosine
Methylthioadenosine phosphorylase
Purine synthesis de novo
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 12 Jul 2016, 12:56:53 EST by System User