Evaluation of antigen-antibody affinity constants by partition equilibrium studies with a two-phase aqueous polymer system: a more rigorous analysis

Winzor D.J. (1991) Evaluation of antigen-antibody affinity constants by partition equilibrium studies with a two-phase aqueous polymer system: a more rigorous analysis. BBA - General Subjects, 1115 2: 141-144. doi:10.1016/0304-4165(91)90022-9


Author Winzor D.J.
Title Evaluation of antigen-antibody affinity constants by partition equilibrium studies with a two-phase aqueous polymer system: a more rigorous analysis
Journal name BBA - General Subjects   Check publisher's open access policy
ISSN 0304-4165
Publication date 1991-12-06
Sub-type Article (original research)
DOI 10.1016/0304-4165(91)90022-9
Open Access Status
Volume 1115
Issue 2
Start page 141
End page 144
Total pages 4
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A quantitative procedure is devised for the determination of affinity constants from the composition-dependence of the antigen partition coefficient in liquid-liquid partition studies of antigen-antibody mixtures. Its use is illustrated by application to published results (Elling, L.R., Kula, M.-R., Hadas, E. and Katchalski-Katzir, E. (1991) Anal. Biochem. 192, 74-77) obtained for the interaction of horseradish peroxidase with an elicited monoclonal antibody by antigen distribution studies with a two-phase aqueous polymer system (poly(ethylene glycol)/dextran) for the partition analysis. Shortcomings in the original interpretation of these results are eliminated.
Keyword Antigen-antibody affinity constant
Horseradish peroxidase
Liquid-liquid partition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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