Changes in the cytoplasmic elements of cultured cells infected with Eimeria vermiformis sporozoites.

Adams J.H. and Bushell G.R. (1989) Changes in the cytoplasmic elements of cultured cells infected with Eimeria vermiformis sporozoites.. Journal of Protozoology, 36 2: 133-138. doi:10.1111/j.1550-7408.1989.tb01059.x


Author Adams J.H.
Bushell G.R.
Title Changes in the cytoplasmic elements of cultured cells infected with Eimeria vermiformis sporozoites.
Journal name Journal of Protozoology   Check publisher's open access policy
ISSN 0022-3921
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1111/j.1550-7408.1989.tb01059.x
Open Access Status Not yet assessed
Volume 36
Issue 2
Start page 133
End page 138
Total pages 6
Language eng
Subject 2405 Parasitology
Abstract Epithelial‐type (PK‐15) and fibroblast‐type (MDBK) mammalian cell cultures were inoculated with purified Eimeria vermiformis sporozoites. Matched samples from 0 to 93 h after inoculation (HAI) were processed for electron microscopy; half of the sample preparations were extracted with non‐ionic detergent prior to fixation. Specimens were examined by both transmission and scanning electron microscopy. Numerous sporozoites were attached to the cultured cells from 2 to 93 HAI, usually near the cell periphery. Some host cell microvilli extended up and appeared attached to the sporozoites. Sporozoites fixed during the penetration process were markedly constricted at the site of entry; however, no noticeable changes occurred in the host cell membrane or surface microvilli during sporozoite invasion or in sporozoite‐infected cells. In cells extracted with 1% Triton X‐100, the host cytoskeleton was progressively reorganized about the parasites but changes were limited to the immediate area of the sporozoite. Around resident sporozoites, the cytoskeleton became less dense but also more ordered, which contrasted with adjacent cell areas. Cytoskeletal elements passed both over and under the parasites. The appearance of the cytoskeleton suggested that the host cell formed a loose, basket‐like net of cytoskeletal elements about the parasite. Copyright
Keyword Apicomplexa
cytoskeletonkw fibroblast
parasite
ultrastructure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 12 Jul 2016, 12:28:21 EST by System User