Excretory-secretory antigens from adult Nematospiroides dubius

Monroy F.G., Adams J.H., East I.J. and Dobson C. (1989) Excretory-secretory antigens from adult Nematospiroides dubius. Immunology and Cell Biology, 67 2: 115-120. doi:10.1038/icb.1989.16

Author Monroy F.G.
Adams J.H.
East I.J.
Dobson C.
Title Excretory-secretory antigens from adult Nematospiroides dubius
Journal name Immunology and Cell Biology   Check publisher's open access policy
ISSN 0818-9641
Publication date 1989-01-01
Sub-type Article (original research)
DOI 10.1038/icb.1989.16
Open Access Status Not yet assessed
Volume 67
Issue 2
Start page 115
End page 120
Total pages 6
Language eng
Subject 1307 Cell Biology
1308 Clinical Biochemistry
2403 Immunology
Abstract Adult Nematospiroides dubius excretory-secretory (ES) products were collected from worms cultured in vitro, radiolabelled and separated by sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). The components were characterized and assessed for molecular weight (MW) after autoradiography and gel staining, for antigenicity in immunoblots, for sensitivity to protease enzymes, and for carbohydrate contents. ES contained at least 18 denatured components from MW < 20000 to 200000. At least one of the surface proteins of adults was found with the ES antigens recovered when adults were cultured in vitro. Molecules with MW 200000, 78000 and 60000 were glycoproteins and reacted with immune mouse serum in Western blots. The dominant ES=, MW 60000 component stained with periodic acid-Schiff (PAS), bound lectins with affinity for D-mannose, and was resistant to peptic and tryptic but not V8 protease digestion.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
Version Filter Type
Citation counts: Scopus Citation Count Cited 5 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 12 Jul 2016, 12:24:12 EST by System User