Heterogeneity of lipoprotein B

Cham B.E., Owens P., Roeser H.P., Gaffney T. and Shanley B.C. (1981) Heterogeneity of lipoprotein B. Biochemical and Biophysical Research Communications, 103 1: 196-205. doi:10.1016/0006-291X(81)91679-X

Author Cham B.E.
Owens P.
Roeser H.P.
Gaffney T.
Shanley B.C.
Title Heterogeneity of lipoprotein B
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1981-11-16
Sub-type Article (original research)
DOI 10.1016/0006-291X(81)91679-X
Volume 103
Issue 1
Start page 196
End page 205
Total pages 10
Language eng
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Combined very low and low density lipoproteins were derived from human plasma by polyanion precipitation and the low density lipoprotein fraction (density 1.027-1.050 g/ml) was isolated by sequential ultracentrifugation. When this fraction was applied to Sepharose column chromatography, three lipoproteins were eluted. The first and third peaks were minor components while the second peak represented the bulk of LDL. Further chromatographic and electrophoretic studies indicated that the component representing the second peak was heterogeneous. This component was subsequently delipidated at pH 4 in a quaternary biphasic solvent system. The apoproteins remained soluble after delipidation and were treated with various deaggregating agents. On column isoelectric focusing in the presence of 4 M urea the apoproteins banded as broad overlapping peaks between pH 3 and 7. When hexanol was added to the system, distinct apoprotein subfractions were resolved.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
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