On the immunological inter-relationships of the vertebrate esterases

Haites-Kingsbury N. and Masters C.J. (1972) On the immunological inter-relationships of the vertebrate esterases. BBA - Enzymology, 289 2: 331-346. doi:10.1016/0005-2744(72)90084-8


Author Haites-Kingsbury N.
Masters C.J.
Title On the immunological inter-relationships of the vertebrate esterases
Journal name BBA - Enzymology
ISSN 0005-2744
Publication date 1972-12-07
Sub-type Article (original research)
DOI 10.1016/0005-2744(72)90084-8
Open Access Status DOI
Volume 289
Issue 2
Start page 331
End page 346
Total pages 16
Subject 2700 Medicine
Abstract 1. 1. In order to assess the immunological inter-relationships of the vertebrate esterases, antisera were produced against several purified enzymes from the different esterolytic classifications, namely sheep liver carboxylesterase (carboxylic-ester hydrolase, EC 3.1.1.1.), horse serum cholinesterase (acylcholine acyl-hydrolase, EC 3.1.1.8), ox testis acetylesterase (acetic-ester hydrolase, EC 3.1.1.6) and ox serum arylesterase (acyl-ester hydrolase, EC 3.1.1.2). 2. 2. These antisera were tested for their interactions at the level of enzyme heterogeneity, by gel electrophoresis of a representative range of tissues before and after incubation with the sera, followed by observation of the alteration in the patterns of esterase heteromorphs consequent upon this treatment. 3. 3. From these results, it appears that a close immunological relationship exists between the multiple forms of each individual esterase type within a single species. In the case of the acetylesterases, this immunological relationship extends between the species as well. A close relationship was also observed between chicken liver carboxylesterase and horse serum cholinesterase, and to a lesser degree between these two latter enzymes and sheep liver carboxylesterase. 4. 4. In addition to the electrophoretic studies, complement fixation inhibition assays were carried out between each of the antisera and all the purified antigens. Also, micro-complement fixation assays between the antisera to the chicken liver carboxylesterase and horse serum cholinesterase were effected with all the antigens. These immunological tests with pure enzymes served to verify that a degree of relationship exists between the hose cholinesterase and the chicken carboxylesterase. 5. 5. The significance of these results has been discussed in relation to the phylogenetic variability of the esterases, the complex structural and genetic inter-relationships of these multiple enzyme forms, and the comparative situation in intensively studied isoenzyme systems.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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