Detection of heat-stable enterotoxin genes among Australian Vibrio cholerae O1 strains

Mallard K.E. and Desmarchelier P.M. (1995) Detection of heat-stable enterotoxin genes among Australian Vibrio cholerae O1 strains. FEMS Microbiology Letters, 127 1-2: 111-115. doi:10.1016/0378-1097(95)00046-8


Author Mallard K.E.
Desmarchelier P.M.
Title Detection of heat-stable enterotoxin genes among Australian Vibrio cholerae O1 strains
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 1574-6968
Publication date 1995-03-15
Sub-type Article (original research)
DOI 10.1016/0378-1097(95)00046-8
Volume 127
Issue 1-2
Start page 111
End page 115
Total pages 5
Subject 1311 Genetics
1312 Molecular Biology
2404 Microbiology
2402 Applied Microbiology and Biotechnology
Abstract DNA probes derived from the heat-stable enterotoxin gene of Vibrio cholerae non-O1 (stn), and the cholera toxin gene (etc), were used to screen 199 strains of V. cholerae O1, which were isolated within Australia from 1977-1986. 13 environmental strains isolated from the riverine environment in Southeast Queensland in 1980 and 1981, hybridized with the stn and ctx DNA probes. The concentrated supernatant of 6 of these strains elicited fluid accumulation in the infant mouse assay both before and after heating at 100 °C for 5 min. Genetic relationships among the 13 stn+ strains were studied by a comparison of the rRNA-RFLPs (ribotyping) and by Southern blot analysis with a stn gene probe. The results indicate that there is a clonal relationship among the Australian stn+ strains and that there is an environmental reservoir of stn genes among Australian V. cholerae O1 isolates.
Keyword DNA probe
Heat-stable enterotoxin
NAG-ST
Vibrio cholerae
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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