Pentachlorophenol removal from slightly acidic mineral salts, commercial sand, and clay soil by recovered Arthrobacter strain ATCC 33790

Edgehill R.U. (1994) Pentachlorophenol removal from slightly acidic mineral salts, commercial sand, and clay soil by recovered Arthrobacter strain ATCC 33790. Applied Microbiology and Biotechnology, 41 1: 142-148. doi:10.1007/BF00166097


Author Edgehill R.U.
Title Pentachlorophenol removal from slightly acidic mineral salts, commercial sand, and clay soil by recovered Arthrobacter strain ATCC 33790
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 0175-7598
Publication date 1994-01-01
Sub-type Article (original research)
DOI 10.1007/BF00166097
Volume 41
Issue 1
Start page 142
End page 148
Total pages 7
Publisher Springer-Verlag
Subject 2726 Microbiology (medical)
2404 Microbiology
1502 Banking, Finance and Investment
1305 Biotechnology
Abstract Arthrobacter strain ATCC 33790, a pentachlorophenol (PCP)-metabolizer isolated by the author, has been recovered after 10 years of storage. The freeze-dried preparation grown on half-strength Trypticase Soy Broth adapted to utilize PCP within 1 week. Cultures grown on PCP-nutrient agar were found to utilize PCP in mineral salts medium within 2-3 days. The culture was prepared for continuous growth at pH 6.5 by successive feeding of 100-110 mg solid aliquots of PCP to a 1-l culture initially grown at pH 7.4. Continuous culture growth at pH 6.5 was possible on a mineral salts feed containing 1800 ppm PCP. Continuous cultures grown at pH 6.7 on mineral salts feeds containing 500 and 340 mg PCP/l were especially efficient in removing PCP. Less than 4 mg PCP/l were detected in the effluent at dilution rates near washout. In batch culture studies at pH 6.5 the PCP utilization kinetics were found to be similar at low PCP concentration to those at pH 7.4 for the approximately same inoculum size. Utilization of 35 mg PCP/l was very slow at pH 6.0. Growth rates at pH 6.5 at controlled PCP concentration ranges of 5-35 and 75-115 mg/l were 0.09 h-1 and 0.05 h-1, respectively. The ability of strain ATCC 33790 to utilize PCP in mineral salts media containing naphthalene, methylnaphthalenes, and cresols was examined. Naphthalene, 1-, and 2-methylnaphthalenes at their solubility limit, and o- and m-cresols at 900-1000 mg/l prevented utilization of 80-90 mg PCP/l. PCP was rapidly removed from both commercial sand at 30°C and from clay soil at room temperature. Estimated inoculum sizes of 6.6 × 106, 6.6 × 104, and 656 cells/g were found to be effective in removing approximately half the starting amount of PCP from sand in 3, 19, and 42 h, respectively. Nearly complete disappearance of extractable PCP was observed after 1 day in clay soil inoculated with 6 × 106 cells/g.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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