Hybridoma cells in a protein-free medium within a composite gel perfusion bioreactor

Shen B.Q., Greenfield P.F. and Reid S. (1994) Hybridoma cells in a protein-free medium within a composite gel perfusion bioreactor. Cytotechnology, 16 1: 51-58. doi:10.1007/BF00761779


Author Shen B.Q.
Greenfield P.F.
Reid S.
Title Hybridoma cells in a protein-free medium within a composite gel perfusion bioreactor
Journal name Cytotechnology   Check publisher's open access policy
ISSN 0920-9069
Publication date 1994-01-01
Sub-type Article (original research)
DOI 10.1007/BF00761779
Volume 16
Issue 1
Start page 51
End page 58
Total pages 8
Publisher Kluwer Academic Publishers
Subject 1308 Clinical Biochemistry
1307 Cell Biology
1305 Biotechnology
Abstract A composite gel system has been developed combining the chemical and physical properties of calcium alginate and agarose gels. The results of growing composite gel immobilized hybridoma SPO1 cells in a protein-free medium within a fluidized-bed perfusion bioreactor are presented in this paper. During the continuous operation of this system, the total cell density reached 3.9×107 cells per ml of beads (viability 79.6%). The specific productivity of monoclonal antibody of the immobilized hybridoma cells reached more than 1.5 μg per 106 viable cells per hour, compared with 0.5 for non-immobilized viable cells grown in a one liter agitated bioreactor with the same medium. Significant increases in cell metabolic activities, including substrate utilization and byproduct formation, were also observed. Leaching of materials from the beads was evident and the major fraction of released materials was alginate.
Keyword Composite gel
growth pattern
hybridoma
metabolic activities
perfusion
protein-free medium
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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