Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda: Heligmosomides).

Adams J.H., East I.J., Monroy F., Washington E.A. and Dobson C. (1987) Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda: Heligmosomides).. Journal of Parasitology, 73 6: 1164-1168.

Author Adams J.H.
East I.J.
Monroy F.
Washington E.A.
Dobson C.
Title Stage-specific antigens of Nematospiroides dubius Baylis, 1926 (Nematoda: Heligmosomides).
Journal name Journal of Parasitology   Check publisher's open access policy
ISSN 0022-3395
Publication date 1987-01-01
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 73
Issue 6
Start page 1164
End page 1168
Total pages 5
Language eng
Subject 1100 Agricultural and Biological Sciences
2404 Microbiology
2405 Parasitology
Abstract Antigens were identified from Nematospiroides dubius recovered from outbred Quackenbush mice between 4 and 10 days postinoculation (PI). Parasite surface proteins were radioiodinated and extracts of the whole worms were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and reacted with normal and immune mouse sera followed by an avidin-biotin-peroxidase assay. Antigens ranged between 250,000 and 20,000 molecular weight (MW). A major surface antigen, 60,000 MW, which appeared to be a complex of different antigens, and a 250,000-MW internal antigen were found on fourth-stage (L4) and fifth-stage (L5) larvae 5-10 days PI but not earlier. A group of minor surface antigens (24,000-30,000 MW) were also expressed as larvae molted from L4 to L5, 6 and 7 days PI, but they differed from antigens of similar MW expressed by adult worms. An antigen, 45,000 MW, was detected in worms 5-10 days PI, but it was only expressed on the surface of L5 worms 9 and 10 days PI. We suggest that the antigen(s) common to adults and larvae may account for protective immunity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Created: Sat, 09 Jul 2016, 17:26:40 EST by System User