Cytokine profiles of cells extracted from humans with periodontal diseases

Gemmell E. and Seymour G.J. (1998) Cytokine profiles of cells extracted from humans with periodontal diseases. Journal of Dental Research, 77 1: 16-26. doi:10.1177/00220345980770010101

Author Gemmell E.
Seymour G.J.
Title Cytokine profiles of cells extracted from humans with periodontal diseases
Journal name Journal of Dental Research   Check publisher's open access policy
ISSN 0022-0345
Publication date 1998-01-01
Sub-type Article (original research)
DOI 10.1177/00220345980770010101
Open Access Status Not yet assessed
Volume 77
Issue 1
Start page 16
End page 26
Total pages 11
Publisher SAGE Publications Inc.
Language eng
Subject 3500 Dentistry
Abstract FACS analysis was used to determine the percent interferon (IFN)-gamma-, interleukin (IL)-4-, IL-10-, and CD30-positive T-cells extracted from adult periodontitis (AP) and healthy/gingivitis (H/G) subjects. Additionally, the percentages of IL-1 beta-, IL-10- and IL-12-producing B-cells and macrophages were ascertained. The percent IL-10+ CD8 cells extracted from AP lesions was decreased compared with H/G tissues (p = 0.033), and the percent CD30+ CD4 (p = 0.001) and CD30+ CD8 (p = 0.028) cells was higher in AP than in H/G tissues. The percentages of IL-1 beta + macrophages (p = 0.003) and IL-12+ B-cells (p = 0.034) were both higher in AP lesions than in H/G tissues. The specific effect of Porphyromonas gingivalis on the cytokine profiles of peripheral blood mononuclear cells isolated from P. gingivalis-infected AP and H/G patients was also determined. While there were no significant differences in the percent cytokine-positive T-cells after stimulation with P. gingivalis outer membrane antigens (OM) for 6 days compared with cells incubated in medium only, the percent CD30+ CD4 cells increased significantly (p = 0.047 and p = 0.063 for AP and H/G groups, respectively). There was also an increase in the percent IL-1 beta + B-cells from AP patients (p = 0.029), and the percent IL-12+ monocytes from AP and H/G subjects was higher than the percent IL-12+ B-cells, both after stimulation with P. gingivalis OM (p = 0.005 for the AP group and p = 0.058 and therefore not quite significant for the H/G group) and when incubated in medium alone (p = 0.016 and p = 0.015 for AP and H/G groups, respectively). This study has shown that IL-10+ CD8 cells may be significant in gingival lesions, and that CD30+ T-cells indicative of Th2 or Th0 cells may play a role in progressive periodontal disease. This study has also shown that B-cells produce IL-1 in the gingival lesion and that P. gingivalis may be significant in the induction of B-cell-induced IL-1.
Keyword B-lymphocytes
Periodontal diseases
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
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Citation counts: TR Web of Science Citation Count  Cited 85 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 90 times in Scopus Article | Citations
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