The involvement of histidine in the action of liver carboxylesterases

Willadsen P., de Jersey J. and Zerner B. (1973) The involvement of histidine in the action of liver carboxylesterases. Biochemical and Biophysical Research Communications, 51 3: 620-625. doi:10.1016/0006-291X(73)91359-4

Author Willadsen P.
de Jersey J.
Zerner B.
Title The involvement of histidine in the action of liver carboxylesterases
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 1090-2104
Publication date 1973-04-02
Sub-type Article (original research)
DOI 10.1016/0006-291X(73)91359-4
Volume 51
Issue 3
Start page 620
End page 625
Total pages 6
Language eng
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Chicken and ox liver carboxylesterases are inhibited irreversibly by α-bromoacetophenone. A kinetic analysis of the process indicates that inhibition occurs at the active site by reaction with a group of pKa′ 6.15 which is reactive in the basic form. Using [14C]bromoacetophenone, it has been shown that loss of activity is accompanied by incorporation of one molecule of inhibitor per active site, together with some non-specific reaction. Acid hydrolysis of the inhibited enzyme leads to a number of radioactive products, including 1- and 3-phenacylhistidines. Chicken liver carboxylesterase is irreversibly inhibited by methyl p-nitrobenzenesulfonate. This inhibition is prevented by the presence of the competitive inhibitor, n-propanol.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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Citation counts: Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Sat, 09 Jul 2016, 16:35:19 EST by System User