Early steps in the biosynthesis of MUC2 epithelial mucin in colon cancer cells

McGuckin M.A., Devine P.L. and Ward B.G. (1996) Early steps in the biosynthesis of MUC2 epithelial mucin in colon cancer cells. Biochemistry and Cell Biology, 74 1: 87-93. doi:10.1139/o96-009


Author McGuckin M.A.
Devine P.L.
Ward B.G.
Title Early steps in the biosynthesis of MUC2 epithelial mucin in colon cancer cells
Journal name Biochemistry and Cell Biology   Check publisher's open access policy
ISSN 0829-8211
Publication date 1996-01-01
Year available 1996
Sub-type Article (original research)
DOI 10.1139/o96-009
Volume 74
Issue 1
Start page 87
End page 93
Total pages 7
Place of publication OTTAWA
Publisher NATL RESEARCH COUNCIL CANADA
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
1303 Specialist Studies in Education
1307 Cell Biology
Abstract Expression of the MUC2 mucin has been demonstrated in normal gastrointestinal and respiratory epithelium and in carcinomas of the gastrointestinal and respiratory tracts, breast, ovary, and bladder using RNA probes and (or) monoclonal antibodies reactive with peptide epitopes on the 23 amino acid tandem repeat. Mouse monoclonal antibodies 4F1 and 3A2 were previously obtained by immunization with mucin derived from the LS174T colon cancer cell Line and a KLH conjugate of a synthetic MUC2 VNTR peptide. These antibodies react with distinct epitopes on synthetic VNTR peptides and with normal and malignant epithelial tissues. In the present study, we examined the biosynthesis of MUC2 in LS174T colon cancer cells, using these antibodies to immunoprecipitate labelled mucin. A very high molecular mass protein was immunoprecipitated following 1 min pulse labelling with [H-3]threonine and [H-3]proline. A slight increase in molecular mass was observed over the next 16 min; however, unlike the MUC1 mucin, there was no large difference in apparent molecular mass between the MUC2 protein precursor and fully processed mucin using separation by SDS-PAGE. O-Glycosylation began within 1 h of synthesis of the protein core. Mucin secretion into the culture medium was detected in the 2nd hour following synthesis and was largely completed within 4 h of synthesis. Secreted mucin was far less reactive with these monoclonal antibodies than the precursor protein.
Keyword Biosynthesis
Cancer
Colon
Glycosylation
MUC2
Mucin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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