A two-chain tumour-related suppressor factor specific for a sequence antigen

Koppi-Reynolds T.A. and Halliday W.J. (1984) A two-chain tumour-related suppressor factor specific for a sequence antigen. Immunology Letters, 8 4: 219-225. doi:10.1016/0165-2478(84)90082-8


Author Koppi-Reynolds T.A.
Halliday W.J.
Title A two-chain tumour-related suppressor factor specific for a sequence antigen
Journal name Immunology Letters   Check publisher's open access policy
ISSN 0165-2478
Publication date 1984-01-01
Sub-type Article (original research)
DOI 10.1016/0165-2478(84)90082-8
Volume 8
Issue 4
Start page 219
End page 225
Total pages 7
Subject 2403 Immunology
2723 Immunology and Allergy
Abstract Suppressor factors in the serum of CBA mice bearing transplanted methylcholanthrene-induced tumours suppressed the leukocyte adherence inhibition reaction between tumour-sensitized peritoneal cells and a sequenced antigen, myelin basic protein (MBP). Chromatography on Sephacryl S-200 columns demonstrated suppressive activity in low molecular weight fractions (40-50 kDa) derived from whole serum. The factors were absorbed from serum by MBP, but not by lysozyme, coupled to Sepharose gel. Cleavage with sodium dodecyl sulphate (SDS) and absorption with either MBP or anti-I-J antibody on Sepharose gels yielded inactive supernatants that regained suppressive activity when combined. Elution of these gels with acidic buffer yielded eluates that were inactive alone but suppressive when combined. These results indicate that the tumour-related suppressor factors consist of two chains separable by detergent action; one chain is antigen (MBP)-binding and the other contains an I-J determinant.
Keyword leukocyte adherence inhibition
murine tumours
myelin basic protein
suppressor factors
two-chain molecule
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
 
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Created: Sat, 09 Jul 2016, 16:19:22 EST by System User