Plant regeneration from protoplasts of durum wheat (Triticum durum Desf. cv. D6962)

Yang Y.M., He D.D. and Scott K.J. (1993) Plant regeneration from protoplasts of durum wheat (Triticum durum Desf. cv. D6962). Plant Cell Reports, 12 6: 320-323. doi:10.1007/BF00237427


Author Yang Y.M.
He D.D.
Scott K.J.
Title Plant regeneration from protoplasts of durum wheat (Triticum durum Desf. cv. D6962)
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1007/BF00237427
Open Access Status Not yet assessed
Volume 12
Issue 6
Start page 320
End page 323
Total pages 4
Publisher Springer-Verlag
Subject 1110 Nursing
Abstract Suspension cultures of durum wheat were established from embryogenic callus maintained in liquid medium for 30 months. Protoplasts were readily isolated from the suspension cultures with yields as high as 3 X 107 protoplasts per g fresh weight suspension cells. When incubated in a modified MS medium containing half strength of the macroelements, 5 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.6 M glucose, protoplast-derived cells divided at frequencies ranging from 1.4 to 10.0 %. After transfer to a solid subculture medium, the protoplast-derived colonies formed embryogenic protuberances, from which green plants have been regenerated.
Keyword embryogenesis
liquid callus cultures
plant regeneration
protoplast
suspension cultures
Triticum durum
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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