Enzymatic methods for the determination of l-serine concentration and l-[14C]serine specific radioactivity in blood plasma

Cassady A.I. and Reilly P.E.B. (1981) Enzymatic methods for the determination of l-serine concentration and l-[14C]serine specific radioactivity in blood plasma. Analytical Biochemistry, 118 1: 23-29. doi:10.1016/0003-2697(81)90151-2


Author Cassady A.I.
Reilly P.E.B.
Title Enzymatic methods for the determination of l-serine concentration and l-[14C]serine specific radioactivity in blood plasma
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1981-11-15
Sub-type Article (original research)
DOI 10.1016/0003-2697(81)90151-2
Open Access Status Not yet assessed
Volume 118
Issue 1
Start page 23
End page 29
Total pages 7
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract Methods are desribed for the use of l-serine dehydratase purified from Clostridium acidiurici for the determination of l-serine concentration and l[14C]serine specific radioactivity in sheep plasma. A spectrophotometric assay using this enzyme accurately measured the concentration of l-serine in standard solutions and in a commercially available mixture of amino acids and related compounds. This assay was shown to be suitable for measurement of plasma l-serine concentrations in excess of 30 μm. The reverse isotope dilution method was used for plasma l-[14C]serine specific radioactivity measurements. Carrier l-serine was added to plasma and separated from neutral and anionic compounds using ion-exchange chromatography. The l-serine was then converted to pyruvate with l-serine dehydratase and this was purified as the phenylhydrazone derivative. After recrystallization, drying and weighing, the derivative was assayed for radioactivity. The accuracy of this method was verified by adding l-[U-14C]serine to plasma and comparing the experimentally determined l-[14C]serine specific radioactivity with the calculated value. The method yielded a value which was 98.6 ± 0.8% (5) of this calculated value.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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