Insulin increases cell numbers and morphological development in mouse pre-implantation embryos in vitro

Gardner H.G. and Kaye P.L. (1991) Insulin increases cell numbers and morphological development in mouse pre-implantation embryos in vitro. Reproduction, Fertility and Development, 3 1: 79-91. doi:10.1071/RD9910079


Author Gardner H.G.
Kaye P.L.
Title Insulin increases cell numbers and morphological development in mouse pre-implantation embryos in vitro
Journal name Reproduction, Fertility and Development   Check publisher's open access policy
ISSN 1448-5990
Publication date 1991-01-01
Sub-type Article (original research)
DOI 10.1071/RD9910079
Open Access Status Not Open Access
Volume 3
Issue 1
Start page 79
End page 91
Total pages 13
Subject 1103 Clinical Sciences
1309 Developmental Biology
1311 Genetics
1305 Biotechnology
1312 Molecular Biology
1310 Endocrinology
2743 Reproductive Medicine
Abstract Insulin, alone or in combination with bovine serum albumin (BSA), was investigated for its effects on cell proliferation and on the proportions of mouse pre-implantation embryos reaching compaction and forming blastocysts during culture in a common basal medium in vitro. Insulin promoted cleavage by 16-20% when added to medium for culture of 2-cell embryos to morulae, blastocysts and expanded blastocysts over 24, 48 and 72 h. These effects on cell division were supported by increases of 65-100% and 31-100% in the rates of compaction and blastocyst formation respectively. The results indicate that the receptor responsible for these actions is probably expressed prior to compaction and possibly at the 4-cell stage. Identical responses to 1-7 and 170 nM insulin suggest that the insulin receptor is capable of mediating both of these developmental effects, although similar mediation by an insulin-like growth factor-1 (IGF-1) receptor is not excluded. BSA, normally a component of culture media, promoted cleavage between 24 and 48 h of culture as well as compaction and blastocyst formation at 15 gM (1 g L-1), probably through nutritional support. Compaction appeared to be promoted by some non-specific action of BSA. Blastocysts that had developed in the presence of both 170 nM insulin and 15, itM BSA contained similar numbers of cells to blastocysts that had developed in vivo.
Keyword Cleavage
Mitogen
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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