Quantitative staining of Fraction I protein in polyacrylamide gels using coomassie brilliant blue

Bennett J. and Scott K.J. (1971) Quantitative staining of Fraction I protein in polyacrylamide gels using coomassie brilliant blue. Analytical Biochemistry, 43 1: 173-182. doi:10.1016/0003-2697(71)90122-9


Author Bennett J.
Scott K.J.
Title Quantitative staining of Fraction I protein in polyacrylamide gels using coomassie brilliant blue
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 1096-0309
Publication date 1971-01-01
Sub-type Article (original research)
DOI 10.1016/0003-2697(71)90122-9
Open Access Status Not yet assessed
Volume 43
Issue 1
Start page 173
End page 182
Total pages 10
Subject 1303 Specialist Studies in Education
1304 Biophysics
1312 Molecular Biology
Abstract A convenient procedure for the extraction, purification, and estimation of Fraction I protein, the major soluble protein in the chloroplast, is described. Extraction is quantitative and purification consists of electrophoresis through 7% polyacrylamide gels. The technique for estimation of Fraction I protein in the gels involves staining the protein bands with Coomassie brilliant blue R250 and then scanning the gels with monochromatic light, using a spectrophotometer and a specially made Perspex gel holder. The limitations and advantages of the technique are discussed, and application of the procedure is illustrated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 05 Jul 2016, 15:31:58 EST by System User