Auxotrophic mutants of pseudomonas aeruginosa

Loutit J.S. (1955) Auxotrophic mutants of pseudomonas aeruginosa. Nature, 176 4471: 74-75. doi:10.1038/176074b0


Author Loutit J.S.
Title Auxotrophic mutants of pseudomonas aeruginosa
Journal name Nature   Check publisher's open access policy
ISSN 0028-0836
Publication date 1955-01-01
Sub-type Article (original research)
DOI 10.1038/176074b0
Volume 176
Issue 4471
Start page 74
End page 75
Total pages 2
Subject 1000 General
Abstract AUXOTROPHIC mutants of a strain of Pseudomonos aeruginosa were isolated by Loutit1 following X-ray treatment and using a modification of the penicillin-isolation technique devised by Davis2 and Lederberg and Zinder3. An attempt was made to repeat this work using a new strain of Pseudomonas aeruginosa; but from a series of ten experiments only one auxotrophic mutant was isolated. A further thirty experiments were carried out on this new strain, using ultra-violet irradiation instead of X-rays and employing the same isolation technique. From these thirty experiments only eleven mutants were isolated, representing less than 0.2 per cent of the total colonies examined. Six of the eleven mutants were isolated in one experiment; but five of these had the same nutritional requirements.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Tue, 05 Jul 2016, 14:31:17 EST by System User