Purine synthesis de novo in cultured lymphoblast cells derived from patients with gout

Gordon R.B. and Emmerson B.T. (1987) Purine synthesis de novo in cultured lymphoblast cells derived from patients with gout. Rheumatology International, 7 1: 1-6. doi:10.1007/BF00267335


Author Gordon R.B.
Emmerson B.T.
Title Purine synthesis de novo in cultured lymphoblast cells derived from patients with gout
Journal name Rheumatology International   Check publisher's open access policy
ISSN 0172-8172
Publication date 1987-01-01
Sub-type Article (original research)
DOI 10.1007/BF00267335
Volume 7
Issue 1
Start page 1
End page 6
Total pages 6
Publisher Springer-Verlag
Subject 2745 Rheumatology
Abstract Rates of de novo purine synthesis in lymphoblast cell cultures derived from ten patients with gout were compared with those from control individuals. Since the rate of de novo purine synthesis was dependent on the growth rate of the culture, an assay procedure was developed to account for the variation in lymphoblast growth rates and to permit valid quantitative comparison between purine synthesis in each cell line. Clear differences were demonstrated between the rates of purine synthesis in cells from normal control subjects and those from patients with a deficiency of hypoxanthine-guanine phosphoribosyltransferase activity (HPRT-deficient). Lymphoblasts from the gouty patients showed purine synthesis either within the normal range or intermediate between this and the HPRT-deficient cells. In patients having normal renal function, de novo purine synthesis of lymphoblast cells correlated with the degree of urate production as reflected by the urinary excretion of urate over a 24 h period. Three patients, with demonstrable excessive production of urate in vivo, exhibited increased purine synthesis in lymphoblasts. This increased synthesis did not appear to result from any of the enzyme mutations currently recognized as responsible for abnormal purine metabolism.
Keyword Cultured lymphoblasts
Gout
Purine synthesis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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